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anti synaptophysin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti synaptophysin
    Anti Synaptophysin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+synaptophysin/Synaptophysin+XP+Rabbit+mAb/pm41917012-255-18-21
    Average 96 stars, based on 178 article reviews
    anti synaptophysin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes
    Article Snippet: .. Subsequently, the membrane was washed three times with TBST and incubated with the primary antibody: anti-Synaptophysin (dilution 1:1000, #4329, Cell Signaling, Danvers, MA, USA), prepared according to the manufacturer’s instructions. ..

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes.
    Article Snippet: .. Subsequently, the membrane was washed three times with TBST and incubated with the primary antibody: anti-Synaptophysin (dilution 1:1000, #4329, Cell Signaling, Danvers, MA, USA), prepared according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes
    Article Snippet: .. Subsequently, the membrane was washed three times with TBST and incubated with the primary antibody: anti-Synaptophysin (dilution 1:1000, #4329, Cell Signaling, Danvers, MA, USA), prepared according to the manufacturer’s instructions. ..

    Article Title: GABA B receptor blockade in the dentate gyrus restores glutamatergic synaptic plasticity and hippocampus dependent memory in an AD-like rat model.
    Article Snippet: Synaptic dysfunction driven by glutamate-mediated excitotoxicity is a hallmark of hippocampus-dependent memory impairment in Alzheimer’s disease (AD).. Although GABAergic signaling is known to regulate excitatory/inhibitory (E/I) balance, the precise molecular mechanisms by which GABA and its receptors modulate glutamatergic synaptic plasticity remains incompletely understood.. Here, we investigated the role of GABA and its receptors in the dentate gyrus (DG) of a streptozotocin (STZ) induced rat model with sporadic AD (SAD)-like features. sAD rats exhibited intact emotional and motor functions but showed marked impairments in novel object recognition, Y-maze, and Morris water maze (MWM) performance.

    Article Title: Microglial-Targeted GCPII Inhibition Reverses Neurocognitive Impairment and Synaptic Loss After EcoHIV Infection.
    Article Snippet: .. Overnight incubation at 4 ◦C followed with primary antibodies in blocking buffer: anti-MAP2 (rabbit, 1:500; Millipore, AB5622-I, Burlington, MA, USA; dendrites), anti-synaptophysin (mouse, 1:500; CST, 9020S, Danvers, MA, USA; presynaptic vesicles), anti-Iba1 (rabbit, 1:500; CST, 17198S, Danvers, MA, USA; microglia), anti-GFAP (chicken, 1:500; Abcam, ab4674, Cambridge, UK; astrocytes), and anti-NeuN (rabbit, 1:500; Abcam, ab177487, Cambridge, UK; neurons). .. Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (1:1000; Invitrogen, A11034, Waltham, MA, USA) for MAP2, Iba1, and NeuN; goat anti-mouse Alexa Fluor 647 (1:1000; Invitrogen, A21240, Waltham, MA, USA) for synaptophysin; and goat anti-chicken Alexa Fluor 488 (1:1000; Invitrogen, A11039, Waltham, MA, USA) for GFAP.

    Article Title: Microglial-Targeted GCPII Inhibition Reverses Neurocognitive Impairment and Synaptic Loss After EcoHIV Infection
    Article Snippet: .. Overnight incubation at 4 °C followed with primary antibodies in blocking buffer: anti-MAP2 (rabbit, 1:500; Millipore, AB5622-I, Burlington, MA, USA; dendrites), anti-synaptophysin (mouse, 1:500; CST, 9020S, Danvers, MA, USA; presynaptic vesicles), anti-Iba1 (rabbit, 1:500; CST, 17198S, Danvers, MA, USA; microglia), anti-GFAP (chicken, 1:500; Abcam, ab4674, Cambridge, UK; astrocytes), and anti-NeuN (rabbit, 1:500; Abcam, ab177487, Cambridge, UK; neurons). .. Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (1:1000; Invitrogen, A11034, Waltham, MA, USA) for MAP2, Iba1, and NeuN; goat anti-mouse Alexa Fluor 647 (1:1000; Invitrogen, A21240, Waltham, MA, USA) for synaptophysin; and goat anti-chicken Alexa Fluor 488 (1:1000; Invitrogen, A11039, Waltham, MA, USA) for GFAP.

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes.
    Article Snippet: .. Subsequently, the membrane was washed three times with TBST and incubated with the primary antibody: anti-Synaptophysin (dilution 1:1000, #4329, Cell Signaling, Danvers, MA, USA), prepared according to the manufacturer’s instructions. ..

    Article Title: A myoneural actuator with engineered biophysics for implantable biohybrid systems.
    Article Snippet: NMJ slides (Fig. 2b, Supplementary Fig. 2a, c–e) were incubated with primary antibodies anti-neurofilament 200 at 1:1000 (Sigma-Aldrich catalog #N4142) and anti-vesicular acetylcholine transporter at 1:500 (Milipore catalog #ABN100) overnight at 4 °C83. .. NMJ slides (Supplementary Fig. 2b) were incubated with primary antibodies anti-neurofilament-L at 1:1000 (Cell signaling catalog #2837) and anti-synaptophysin at 1:1000 (Cell signaling catalog #36406) overnight at 4 °C. .. Sequentialmuscles slides for fiber type analysis were incubatedwith primary antibodies anti-myosin fast clone MY-32 at 1:500 (Sigma-Aldrich catalog #M1570) and antimyosin slow clone NOQ7.5.4D at 1:10000 (Sigma Aldrich catalog #M8421) overnight at 4 °C.

    Article Title: A myoneural actuator with engineered biophysics for implantable biohybrid systems
    Article Snippet: Slides were then washed three times with PBS for 5 min. NMJ slides (Fig. , Supplementary Fig. ) were incubated with primary antibodies anti-neurofilament 200 at 1:1000 (Sigma-Aldrich catalog #N4142) and anti-vesicular acetylcholine transporter at 1:500 (Milipore catalog #ABN100) overnight at 4 °C . .. NMJ slides (Supplementary Fig. ) were incubated with primary antibodies anti-neurofilament-L at 1:1000 (Cell signaling catalog #2837) and anti-synaptophysin at 1:1000 (Cell signaling catalog #36406) overnight at 4 °C. .. Sequential muscles slides for fiber type analysis were incubated with primary antibodies anti-myosin fast clone MY-32 at 1:500 (Sigma-Aldrich catalog #M1570) and anti-myosin slow clone NOQ7.5.4D at 1:10000 (Sigma Aldrich catalog #M8421) overnight at 4 °C.

    Article Title: Single-nuclei RNA-sequencing uncovers sexually divergent exercise signatures partially mimicked by TFEB overexpression in mouse skeletal muscle
    Article Snippet: Muscle sections from a second independent cohort of mice were permeabilized with 0.1% Triton (Thermo Scientific, TritonX-100, A16046AE) in 1X PBS for 15min and blocked with 5% BSA for 30min. .. Sections were incubated in primary antibodies: anti-laminin (R&D Systems, MAB2549, 1:100), anti-5HT1B (Millipore Sigma, SAB4501470, 1:200), anti-CD31 (BD Pharmingen, 550274, 1:200), anti-COL6A1 (Proteintech, 17023-1-AP, 1:200), anti-synaptophysin (Cell Signaling Technology, D8F6H, 1:200), and anti-MHC Type 2B (DSHB, BF-F3, 1:100) overnight at 4°C. ..

    Blocking Assay:

    Article Title: Microglial-Targeted GCPII Inhibition Reverses Neurocognitive Impairment and Synaptic Loss After EcoHIV Infection.
    Article Snippet: .. Overnight incubation at 4 ◦C followed with primary antibodies in blocking buffer: anti-MAP2 (rabbit, 1:500; Millipore, AB5622-I, Burlington, MA, USA; dendrites), anti-synaptophysin (mouse, 1:500; CST, 9020S, Danvers, MA, USA; presynaptic vesicles), anti-Iba1 (rabbit, 1:500; CST, 17198S, Danvers, MA, USA; microglia), anti-GFAP (chicken, 1:500; Abcam, ab4674, Cambridge, UK; astrocytes), and anti-NeuN (rabbit, 1:500; Abcam, ab177487, Cambridge, UK; neurons). .. Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (1:1000; Invitrogen, A11034, Waltham, MA, USA) for MAP2, Iba1, and NeuN; goat anti-mouse Alexa Fluor 647 (1:1000; Invitrogen, A21240, Waltham, MA, USA) for synaptophysin; and goat anti-chicken Alexa Fluor 488 (1:1000; Invitrogen, A11039, Waltham, MA, USA) for GFAP.

    Article Title: Microglial-Targeted GCPII Inhibition Reverses Neurocognitive Impairment and Synaptic Loss After EcoHIV Infection
    Article Snippet: .. Overnight incubation at 4 °C followed with primary antibodies in blocking buffer: anti-MAP2 (rabbit, 1:500; Millipore, AB5622-I, Burlington, MA, USA; dendrites), anti-synaptophysin (mouse, 1:500; CST, 9020S, Danvers, MA, USA; presynaptic vesicles), anti-Iba1 (rabbit, 1:500; CST, 17198S, Danvers, MA, USA; microglia), anti-GFAP (chicken, 1:500; Abcam, ab4674, Cambridge, UK; astrocytes), and anti-NeuN (rabbit, 1:500; Abcam, ab177487, Cambridge, UK; neurons). .. Secondary antibodies included goat anti-rabbit Alexa Fluor 488 (1:1000; Invitrogen, A11034, Waltham, MA, USA) for MAP2, Iba1, and NeuN; goat anti-mouse Alexa Fluor 647 (1:1000; Invitrogen, A21240, Waltham, MA, USA) for synaptophysin; and goat anti-chicken Alexa Fluor 488 (1:1000; Invitrogen, A11039, Waltham, MA, USA) for GFAP.



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    Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) <t>Synaptophysin,</t> positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.
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    Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) <t>Synaptophysin,</t> positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.
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    Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) <t>Synaptophysin,</t> positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.
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    Image Search Results


    Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) Synaptophysin, positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.

    Journal: Oncology Letters

    Article Title: Proliferative escalation and possible neuroendocrine carcinoma transformation in pulmonary metastases of a cervical neuroendocrine tumor: A case report

    doi: 10.3892/ol.2026.15501

    Figure Lengend Snippet: Histopathological and immunohistochemical findings of the cervical tumor. (A) H&E staining (ocular, ×10; objective, ×4; final magnification, ×40) showing mixed histology. (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100) revealing coexistence of adenocarcinoma and neuroendocrine components. (C) H&E staining of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400). (D-L) Immunohistochemical analysis of the neuroendocrine component (ocular, ×10; objective, ×40; final magnification, ×400) revealing the following: (D) Synaptophysin, positive; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; (K) Ki-67, low proliferative activity; and (L) Ki-67, hotspot with high proliferative activity.

    Article Snippet: The primary antibodies used for the immunostaining shown in and were as follows: synaptophysin (catalog no. 413831, clone 27G12; Nichirei Biosciences), chromogranin A (catalog no. M086901-2, clone DAK-A3; DAKO), p16 (catalog no. 550834, clone G175-405; BD Biosciences), YAP1 (catalog no. ab52771, clone EPR1674Y; Abcam), TTF-1 (catalog no. NCL-L-TTF-1, clone SPT24; NOVO), retinoblastoma protein (Rb; catalog no. 554136, clone G3-245; BD Biosciences), PAX8 (catalog no. ab53490, clone PAX8R1; abcam), and Ki-67 (catalog no. M7240, clone MIB-1; DAKO).

    Techniques: Immunohistochemical staining, Staining, Expressing, Activity Assay

    Histopathological and immunohistochemical findings of the left upper lobe lung tumor. (A) Low-power view of H&E staining (scale bar, 2.5 mm). (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100). (C) H&E staining (ocular, ×10; objective, ×20; final magnification, ×200). (A-C) The tumor consisted predominantly of NET components but with areas of increased chromatin and a higher nuclear-to-cytoplasmic ratio, suggesting the presence of NEC components. (D-K) Immunohistochemical analysis (ocular, ×10; objective, ×10; final magnification, ×100) revealed the following: (D) Synaptophysin, positive in NET areas but weaker in suspected NEC areas; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative in NET areas but focally positive in suspected NEC areas; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; and (K) Ki-67, high proliferative index in suspected NEC areas. NEC, neuroendocrine carcinoma; NET, neuroendocrine tumor.

    Journal: Oncology Letters

    Article Title: Proliferative escalation and possible neuroendocrine carcinoma transformation in pulmonary metastases of a cervical neuroendocrine tumor: A case report

    doi: 10.3892/ol.2026.15501

    Figure Lengend Snippet: Histopathological and immunohistochemical findings of the left upper lobe lung tumor. (A) Low-power view of H&E staining (scale bar, 2.5 mm). (B) H&E staining (ocular, ×10; objective, ×10; final magnification, ×100). (C) H&E staining (ocular, ×10; objective, ×20; final magnification, ×200). (A-C) The tumor consisted predominantly of NET components but with areas of increased chromatin and a higher nuclear-to-cytoplasmic ratio, suggesting the presence of NEC components. (D-K) Immunohistochemical analysis (ocular, ×10; objective, ×10; final magnification, ×100) revealed the following: (D) Synaptophysin, positive in NET areas but weaker in suspected NEC areas; (E) chromogranin A, positive; (F) p16, positive; (G) yes-associated protein 1, negative; (H) thyroid transcription factor-1, negative in NET areas but focally positive in suspected NEC areas; (I) retinoblastoma protein, wild-type expression; (J) paired box 8, negative; and (K) Ki-67, high proliferative index in suspected NEC areas. NEC, neuroendocrine carcinoma; NET, neuroendocrine tumor.

    Article Snippet: The primary antibodies used for the immunostaining shown in and were as follows: synaptophysin (catalog no. 413831, clone 27G12; Nichirei Biosciences), chromogranin A (catalog no. M086901-2, clone DAK-A3; DAKO), p16 (catalog no. 550834, clone G175-405; BD Biosciences), YAP1 (catalog no. ab52771, clone EPR1674Y; Abcam), TTF-1 (catalog no. NCL-L-TTF-1, clone SPT24; NOVO), retinoblastoma protein (Rb; catalog no. 554136, clone G3-245; BD Biosciences), PAX8 (catalog no. ab53490, clone PAX8R1; abcam), and Ki-67 (catalog no. M7240, clone MIB-1; DAKO).

    Techniques: Immunohistochemical staining, Staining, Expressing